Arduino PCR (thermal cycler) for under $85

Fmxhyhah4Agjrqd.Large

Arduino PCR (thermal cycler) for under $85.

This tutorial will show you how to make a thermal cycler from scratch for about $85. In short, PCR(polymerase chain reaction) amplifies bits of DNA, creating millions of copies of a target sequence. You can use it to test a DNA sample for a specific gene, for instance, to check for genetic modification in food and for hereditary gene testing.

During PCR, a mixture of DNA, primer and DNA polymerase is cycled between three different temperature settings, over and over again. This project uses an arduino to control two high-power resistors to heat up the sample, a computer fan to cool down, and a thermocouple to keep track of the temperature. The design supports two samples at a time, though it could probably be extended to support more.

The parts are all off-the shelf, and the assembly should take a few hours. You will need access to a shop (at the very least a ban saw and drill press).


Eink, E-paper, Think Ink – Collin shares six segments pondering the unusual low-power display technology that somehow still seems a bit sci-fi – http://adafruit.com/thinkink

Stop breadboarding and soldering – start making immediately! Adafruit’s Circuit Playground is jam-packed with LEDs, sensors, buttons, alligator clip pads and more. Build projects with Circuit Playground in a few minutes with the drag-and-drop MakeCode programming site, learn computer science using the CS Discoveries class on code.org, jump into CircuitPython to learn Python and hardware together, TinyGO, or even use the Arduino IDE. Circuit Playground Express is the newest and best Circuit Playground board, with support for CircuitPython, MakeCode, and Arduino. It has a powerful processor, 10 NeoPixels, mini speaker, InfraRed receive and transmit, two buttons, a switch, 14 alligator clip pads, and lots of sensors: capacitive touch, IR proximity, temperature, light, motion and sound. A whole wide world of electronics and coding is waiting for you, and it fits in the palm of your hand.

Join 26,000+ makers on Adafruit’s Discord channels and be part of the community! http://adafru.it/discord

Have an amazing project to share? The Electronics Show and Tell is every Wednesday at 7pm ET! To join, head over to YouTube and check out the show’s live chat – we’ll post the link there.

Join us every Wednesday night at 8pm ET for Ask an Engineer!

Follow Adafruit on Instagram for top secret new products, behinds the scenes and more https://www.instagram.com/adafruit/

CircuitPython – The easiest way to program microcontrollers – CircuitPython.org


Maker Business — To make it through a tough business cycle, layoffs should be a last resort

Wearables — Be outdoor ready

Electronics — When do I use X10?

Python for Microcontrollers — Python on Microcontrollers Newsletter: CircuitPython 6.1.0.RC0, Blinka Explained, and more! #Python #Adafruit #CircuitPython @micropython @ThePSF

Adafruit IoT Monthly — Our Favorite IoT Projects from 2020

Microsoft MakeCode — Space Jam, The MakeCode Show & More!

EYE on NPI — Maxim’s Himalaya uSLIC Step-Down Power Module #EyeOnNPI @maximintegrated @digikey

New Products – Adafruit Industries – Makers, hackers, artists, designers and engineers! — NEW PRODUCTS – Ultra Flexible White LED Strips – 480 per meter – 5m long – Warm White + Cool White

Get the only spam-free daily newsletter about wearables, running a "maker business", electronic tips and more! Subscribe at AdafruitDaily.com !



No Comments

No comments yet.

Sorry, the comment form is closed at this time.