Microscopes make living cells and tissues appear bigger. But what if we could actually make the things bigger?
It might sound like the fantasy of a scientist who has read Alice’s Adventures in Wonderland too many times, but the concept is the basis for a new method that could enable biologists to image an entire brain in exquisite molecular detail using an ordinary microscope, and to resolve features that would normally be beyond the limits of optics.
The technique, called expansion microscopy, involves physically inflating biological tissues using a material more commonly found in baby nappies (diapers). Edward Boyden, a neuroengineer at the Massachusetts Institute of Technology (MIT) in Cambridge, discussed the technique, which he developed with his MIT colleagues Fei Chen and Paul Tillberg, at a conference last month.
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