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The majority of microfluidic applications require an external pumping mechanism. Multi-channel, individually addressable pumps are expensive, often large, and prone to failure when operated inside cell culture incubators at 95% humidity. The number of experiments that can be run at a given time is limited by the availability and expense of pumps. Perfusing artificial tissue scaffolds containing engineered vasculature requires long-term (days to weeks) continuous flow at low rates. We designed an inexpensive (~$100 for 2 pumps, ~$70 for each additional set of 2 pumps) peristaltic pumping system using an Arduino- controlled stepper motor fitted with a custom 3D-printed pump head and laser-cut mounting bracket. Each pump has a footprint roughly that of the NEMA 17 stepper motor and is easily controlled individually using open source software. Up to 64 motor shields can be stacked for a given Arduino Uno R3, each capable of supporting two stepper motors, and thus has the expansion potential to control 128 pumps in parallel. We have successfully implemented two stacked motor shields driving four independent stepper motors. Flow rate is dependent upon both tubing diameter and step rate. We found flow rates to range between ~50-250 μl/min for 1/16” tubing and ~500-1500 μl/min for 1/4″ tubing. We anticipate that this pump design will likely prove more resilient to incubator humidity compared to standard peristaltic pump powered by DC motors. Since implementation, these pumps have functioned without fail for 3 months (intermittent) under humid conditions. In the event of failure, however, cost of motor replacement is an economical $14.
Featured Adafruit Products!
Adafruit Motor/Stepper/Servo Shield for Arduino v2 Kit – v2.3: The original Adafruit Motorshield kit is one of our most beloved kits, which is why we decided to make something even better. We have upgraded the shield kit to make the bestest, easiest way to drive DC and Stepper motors. This shield will make quick work of your next robotics project! We kept the ability to drive up to 4 DC motors or 2 stepper motors, but added many improvements:
Instead of a L293D darlington driver, we now have the TB6612 MOSFET driver: with 1.2A per channel and 3A peak current capability. It also has much lower voltage drops across the motor so you get more torque out of your batteries, and there are built-in flyback diodes as well. Read more.
Stepper motor – NEMA-17 size – 200 steps/rev, 12V 350mA: A stepper motor to satisfy all your robotics needs! This 4-wire bipolar stepper has 1.8° per step for smooth motion and a nice holding torque. The motor was specified to have a max current of 350mA so that it could be driven easily with an Adafruit motor shield for Arduino (or other motor driver) and a wall adapter or lead-acid battery. Read more.
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