Over the course of their careers, biologists develop a huge mental library of cell structures and their corresponding data. Investigating specific areas of a living cell involves a piecemeal approach, identifying how some parts work with others and spending time on cell labelling. But now, the Allen Institute for Cell Science has launched the first predictive 3D model of a live human cell — the Allen Integrated Cell — and it could be “a total game changer”, according to researchers.
“This is a new way to see inside living human cells,” said Rick Horwitz, executive director of the Allen Institute for Cell Science. “It’s like seeing the whole cell for the first time. In the future, this will impact drug discovery, disease research and how we frame basic studies involving human cells.”
To create the breakthrough model, scientists gene edited a large collection of live human cells to incorporate fluorescent protein tags, which illuminate specific structures inside the cells. The team took tens of thousands of pictures of these glowing cells, and used AI to create a probabilistic model, which predicts the most probable shape and location of structures in any cell, based on the shape of the plasma membrane and the nucleus.
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