Researching Histone H3 Using CRISPR-Cas9-Mediated Gene Editing

The Johns Hopkins News-Letter explains how university researchers identified the role of Histone H3 in regulating cell plasticity in worm embryos using CRISPR-Cas9-mediated gene editing.

The researchers found that increasing the H3 level was associated with decreased cellular plasticity of pluripotent cells in worm embryos. Pluripotent cells are cells before differentiation that have the potential to become specialized cells, such as neurons and muscle cells. When the H3 level was reduced, the duration of pluripotency for cells in worm embryos was extended. Based on this, the researchers concluded that modifying the H3 level can regulate normal embryogenesis pathways and make the embryo cells adopt an alternative cell fate.

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